M-----R---C--DRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTFRG------------------------HTAAFIDLSGP-------KDE
MKPVHARVRLCQDDRDIWAWLDEGRLSINPRPPVERINGATVTVRLGNKFDWFVGNRFWMFNVVEIRTNNKEIGSWFRGHTAADIDDSGPCKAEEKMKDE
+-----+---+--++++.++++++++++++++++++++++++.+++++++..+.+------------------------++++.++.+++-------+++

M=52
X=7
G=41
W = 52-7-41=4